CytoSure array and NGS products for cytogenetics and rare disease
CytoSure® is a collection of products that have been developed with input from leaders in the fields of cytogenetics, cancer and rare disease research – which have been optimised to provide the most relevant content and sensitivity.
OGT has over two decades' experience in the development of microarrays and we have used that experience to provide CytoSure arrays for investigating a large range of disease phenotypes, with excellent CNV and LOH calling, down to the exon level.
CytoSure has expanded to include NGS platforms for cytogenetic and rare disease research that have the same superior CNV calling as the arrays but also the added benefit of SNV and Indel calling.
Both the arrays and NGS platforms are supported by complimentary analytical software, CytoSure Interpret and Interpret, respectively. These software packages have been developed at OGT and have been designed to be adaptable and user-friendly.
A highly targeted exon-focused array capable of detecting medically relevant microdeletions and microduplications. This array has been developed in collaboration with leading molecular genetics experts at Emory University and makes an ideal complement to an exome sequencing approach, providing a comprehensive mutation spectrum analysis in rare disease. The figure (above) shows [A] a small duplication of 1.4kb in the DMD gene and [B] a very small 684bp deletion in the TRPM1 gene.
View ProductOGT’s range of CytoSure® Cancer +SNP arrays combine long-oligo probes for superior copy number variant (CNV) detection alongside single nucleotide polymorphism (SNP) probes – which function using OGT proprietary technology – for accurate identification of loss-of-heterozygosity (LOH). The figure (above) shows a schematic of OGT’s unique SNP probe technology, allowing the use of any reference sample with no restriction digest.
View ProductA targeted >700-gene panel, minimising variants of uncertain significance (VUS) detection, and delivering CNV analysis down to single-exon level and loss of heterozygosity (LOH) as well as SNV and indel detection, all in a single assay. The figure (above) shows a 3.98Mb deletion on chromosome 6. The B allele plot (Panel 1) and the CNV ratio result (Panel II) are both shown.
View ProductFamilial Hypercholesterolaemia (FH) is a genetic condition which results in a high cholesterol level and subsequently leads to a higher risk of early heart disease. It affects approximately 1 in 250 people with around 34 million cases worldwide. OGT is offering an optimised NGS panel which has selected the most relevant genes and SNPs implicated in FH, for your research needs. The figure (above) shows a double deletion on the LDLR gene, as visualised by Interpret software.
View ProductThe labelling of DNA samples used in aCGH is a critical step in the experimental process, as poor labelling can result in inaccurate data. OGT’s labelling kits have been uniquely developed and optimised to enable rapid delivery of high-quality results with high signal-to-noise ratios. The figure (above) shows that the DNA labelled using CytoSure Genomic Labelling Kit exhibited far superior Cy3 signal intensity compared to another commercially available kit.
View ProductA powerful and easy-to-use package for the analysis of aCGH data, offering an impressive combination of features that allow you the choice of standardised data analysis (using the Accelerate Workflow) or customised, user-defined data analysis. The figure (above) shows a gain on chromosome 12 for a CLL sample, which contains the zinc finger protein gene ZP384, easily identified in the Cancer gene census genes track.
View ProductOur latest and most advanced system for capture of targeted genomic regions and generation of NGS libraries. Delivering a 40% reduction in hands-on time compared to the previous workflow, increased accuracy and error correction with the inclusion of UDIs and UMIs and reduced workflow complexity for minimal risk of human error. The figure (above) shows the workflow.
View ProductA powerful and easy-to-use NGS analysis solution, facilitating analysis and visualisation of a wide range of variants and structural aberrations. Coupled with a comprehensive and powerful filtering framework, the software delivers accurate calling of SNVs and indels, as well as structural aberrations, including ITDs, PTDs, CNVs, LOH and translocations. The figure (above) shows detection of a KMT2A-PTD spanning exons 2-8.
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