Improving experimental reproducibility through automated hybridisation-based NGS library preparation

Introduction

One of the greatest challenges for laboratories running next generation sequencing (NGS) panels is to ensure high quality, reproducible results whilst sustaining high sample throughput in a timely fashion. Automation enables standardisation of OGT's SureSeq™ NGS library preparation, hybridisation and washing for up to 96 samples in a single batch, improving laboratory productivity. In this application note, an Agilent Bravo^® A Automated Liquid Handling Platform was configured to run the SureSeq NGS library preparation protocol. The results demonstrate marked improvement not only in hands-on-time, but also a number of quality metrics, in particular, reduced variance in % on-target reads when compared to manual processing.