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Introduction

One of the greatest challenges for laboratories running next generation sequencing (NGS) panels is to ensure high quality, reproducible results whilst sustaining high sample throughput in a timely fashion. Automation enables standardization of OGT's SureSeq™ NGS library preparation, hybridization and washing for up to 96 samples in a single batch, improving laboratory productivity. In this application note, an Agilent Bravo® A Automated Liquid Handling Platform was configured to run the SureSeq NGS library preparation protocol. The results demonstrate marked improvement not only in hands-on-time, but also a number of quality metrics, in particular, reduced variance in % on-target reads when compared to manual processing.

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