First slide
Cytocell Logo

Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Prostate cancer
  • Region 21q22.2-q22.3
    21q22.13-q22.2
  • Label      
  • Product No. LPS 021 (10 tests)
    LPS 021-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • TMPRSS2, 21q22.2-q22.3, Red
  • TMPRSS2, 21q22.2-q22.3, Green
  • ERG, 21q22.13-q22.2, Blue

The TMPRSS2/ERG Deletion/Breakapart probe is a three colour probe that consists of green probes and red probes positioned on each side of the TMPRSS2 gene, as well as three blue probes that cover the centromeric region of the ERG gene, up to D21S1439 marker.

 

Probe information

Recent studies suggest that over 50% of prostate tumours may carry a chromosomal rearrangement of chromosome 21q221. The genes involved are the androgen-inducible TMPRSS2 (Transmembrane protease, serine 2 (21q22.3)) and an ETS (E-twenty six) family transcription factor, ERG (v-ets erythroblastosis virus E26 oncogene like (21q22.2))1.

An apparent intrachromosomal deletion of 3Mb on chromosome 21 fuses TMPRSS2 to ERG. The rearrangement places ERG under the androgen-regulated transcriptional control of TMPRSS22. This fusion is associated with disease progression and the rearrangement in prostate cancer may account for molecular and clinical heterogeneity3. This represents an important subclassification of prostate cancer from a biological and a clinical standpoint3.

What our customers say

References

  1. Saramaki et al., Clin Cancer Res 2008;14(11):3395-3400
  2. Lapointe et al., Modern Pathology 2007;20(4):467-473
  3. Perner et al., Cancer Res 2006;66(17):8337-8341

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
Play Icon

Solid tumour FFPE FISH protocol

Request a quote for this product

FISH resources & support