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Product summary

  • Technology FISH
  • Application Solid tumour
  • Product No. LPS 100
  • Intended use For use in heat pretreatment and enzyme digestion of Formalin-Fixed, Paraffin Embedded (FFPE) tissue prior to Fluorescence in situ Hybridisation (FISH) or Chromogenic in situ Hybridisation (CISH) detection. This product is intended for general laboratory use or use in a Laboratory Developed Test (LDT). If used as part of an LDT, it is the responsibility of the laboratory developing the test to validate the test before use in a clinical setting.

Overview

Introducing the first Pretreatment kit capable of preparing slides for CISH and/or FISH analysis on Formalin-Fixed, Paraffin-Embedded (FFPE) tissue.

Our ready-to-use Tissue Pretreatment Kit has been optimised to produce excellent visual results with our extensive CytoCell® Pathology FISH range.

To further extend the utility of the kit we have also validated its use with other commercially available CISH (chromogenic in situ hybridisation) and FISH (fluorescence in situ hybridisation) DNA probes*.

With ease-of-use and convenience in mind, our simple two stage FFPE slide preparation protocol employs the use of ready-to-use reagents; which have been optimised to increase the permeabilisation of cell membranes and facilitate penetration of the desired FISH or CISH DNA probe.

 

LABEL LICENSE - This product is provided under an agreement between Life Technologies Corporation and Cytocell Ltd. Purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product only for life science research or human diagnostics, as set forth in the accompanying product literature. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment other than human diagnostics testing services; (3) for therapeutic or prophylactic purposes:(4) to resell, sell or otherwise transfer this product or its components to any third party, or use for any use other than use in life science research or human diagnostics, whether or not such product or its components are resold for use in research or human diagnostics. For information about obtaining rights for use of this product for any other purpose, contact outlicensing@lifetech.com.

* A list of manufacturers is available upon request.

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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