Introducing the first Pretreatment kit capable of preparing slides for CISH and/or FISH analysis on Formalin-Fixed, Paraffin-Embedded (FFPE) tissue.
Our ready-to-use Tissue Pretreatment Kit has been optimised to produce excellent visual results with our extensive CytoCell® Pathology FISH range.
To further extend the utility of the kit we have also validated its use with other commercially available CISH (chromogenic in situ hybridisation) and FISH (fluorescence in situ hybridisation) DNA probes*.
With ease-of-use and convenience in mind, our simple two stage FFPE slide preparation protocol employs the use of ready-to-use reagents; which have been optimised to increase the permeabilisation of cell membranes and facilitate penetration of the desired FISH or CISH DNA probe.
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* A list of manufacturers is available upon request.
It has saved us an incredible amount of time; now the entire pretreatment process, including probing, takes just three hours rather than a full day, which has increased our capacity. We no longer have to repeat 50% of our samples, more like 5%.
Assistant-Chief Medical Technologist, Core Molecular Diagnostic Laboratory-Cytogenetics, McGill University Health Centre, Montreal, Quebec, Canada