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Product summary

  • Technology FISH
  • Application Solid tumour
  • Product No. LPS 100*
  • Intended use This product is provided under an agreement between Life Technologies Corporation and Cytocell Ltd and is available for human diagnostics or life sciences use only.

Overview

Introducing the first Pretreatment kit capable of preparing slides for CISH and/or FISH analysis on Formalin-Fixed, Paraffin-Embedded (FFPE) tissue.

Our ready-to-use Tissue Pretreatment Kit has been optimised to produce excellent visual results with our extensive CytoCell® Pathology FISH range.

To further extend the utility of the kit we have also validated its use with other commercially available CISH (chromogenic in situ hybridisation) and FISH (fluorescence in situ hybridisation) DNA probes**.

With ease-of-use and convenience in mind, our simple two stage FFPE slide preparation protocol employs the use of ready-to-use reagents; which have been optimised to increase the permeabilisation of cell membranes and facilitate penetration of the desired FISH or CISH DNA probe.

 

* This product is provided under an agreement between Life Technologies Corporation and Cytocell Ltd and is available for human diagnostics or life science use only.

** A list of manufacturers is available upon request.

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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