First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma, Brain tumour, Lung cancer
  • Region 9p21.3
    9q12
  • Label    
  • Product No. LPS 036 (10 tests)
    LPS 036-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • P16, 9p21.3, Red
  • D9Z3, 9q12, Green

The P16 probe, labelled in red, covers a 193kb region of 9p21.3, extending from 105kb telomeric of P16 (CDKN2A) gene to 46kb centromeric of CDKN2B. The probe mix also contains a control probe for chromosome 9 (D9Z3, the heterochromatic block at 9q12) labelled in green.

 

Probe information

Diffuse large B-cell lymphoma (DLBCL) is the most frequent form of aggressive lymphoma and deletions of the P16 (now known as CDKN2A) locus occur in approximately one-third of DLBCL patients1.

The CDKN2A gene encodes a cyclin dependent kinase inhibitor that functions in the control of phosphorylation of Retinoblastoma (RB), a protein that is frequently hypermethylated in lymphomas2. There are two proteins produced by the CDKN2A gene, P16 and P14ARF, the latter being an alternate reading frame product. These protein products have been linked to two tumour suppressor pathways, the RB pathway and the p53 pathway. P16 inhibits phosphorylation of RB, whereas P14ARF targets MDM2, a P53 inhibitory protein, for degradation. A deletion of the CDKN2A gene would therefore disturb both pathways3.

Losses of CDKN2A are significantly associated with a shorter survival after treatment4.

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References

  1. Guney et al., Genes Chromosomes Cancer. 2012 Sep;51(9):858-67
  2. Chim et al., Hum Pathol. 2007 Dec;38(12):1849-57
  3. Møller et al., Leukemia. 1999 Mar;13(3):453-9
  4. Jardin et al., Blood. 2010 Aug 19;116(7):1092-104

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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