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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 22q11.21-q11.23
  • Label    
  • Product No. LPS 039 (10 tests)
    LPS 039-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGL, 22q11.21-q11.23, Red
  • IGL, 22q11.21-q11.23, Green

The IGL product consists of a 278kb probe, labelled in red, centromeric to the IGL Variable region and covering the MAPK1 gene, and a green probe, covering a 307kb region telomeric to the IGL Constant segment, including the BCR gene.

 

Probe information

Translocations involving the immunoglobulin loci are recurring events in various subtypes of B-cell lymphomas.

In addition to translocations involving the IGH locus, variant translocations have been described in 5-10% of B-cell neoplasms involving either the immunoglobulin kappa (IGK) light chain locus at 2p11.2 or the lambda light chain (IGL) at 22q111,2. The best known translocations involving IG light chain loci are the variant Burkitt's translocations t(2;8)(p12;q24) and t(8;22)(q24;q11) present in up to 21% of all Burkitt's lymphomas3.

Other translocations involve the BCL6 oncogene, the t(2;3)(p12;q27) and t(3;22)(q27;q11) and BCL2 locus, t(2;18)(p12;q21) and t(18;22)(q21;q11)4,5. Translocations involving the IG light chain loci usually lead to breakage within the joining region of the respective locus2. IGL consists of 38 potentially active variable (IGLV) gene segments, 35 pseudogenes and seven IGL constant gene segments, each with a joining (J)-segment IGL (J-C)1.

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References

  1. Poulseu TS et al., Leukemia 2002;16:2148-55
  2. Martin-Subero JI et al., Int J Cancer 2002;98:470-4
  3. Kornblau SM et al., Hematol Oncol 1991;9:63-78
  4. Chaganti SR et al., Genes Chromosomes Cancer 1998;23:323-7
  5. Tashiro S et al., Oncogene 1992;7:573-7

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.

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