Tips include how to minimise duplication rates in sequencing results.
After adaptor ligation, use the lowest cycle number possible based on the DNA concentration. This will minimise duplication rates in sequencing results.
NGS Field Application Scientist, North America
Figure 1: Analysis of amplified prepared library DNA using an Agilent D1000 ScreenTape assay. The electropherogram shows a single peak in the size range of 200 to 275 bp (+/-10%).