Tips include advice for washes and how to prevent less efficient washing of the DNA hybridised to the streptavidin beads.
Washes must be pre-warmed as per the handbook.
Pete Gray
Array and NGS Field Application Specialist, Europe
Test thermocyclers and consumables to be used during hybridisation and wash steps if using alternatives to minimise evaporation. Evaporation of the wash buffers alters the ratio of buffer:sample. This may lead to less efficient washing of the DNA hybridised to the streptavidin beads.
Huiyan Jin
NGS Field Application Scientist, North America
Figure 1: Comparison of Hybridisation Wash temperatures showing minimum coverage over the 7 gene targets in the Ovarian panel. Washing at 40˚C gives the best results.
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