Tips include why should you prepare the reaction on ice and advice on how to heat samples prior to adaptor ligation.
Prepare reaction on ice since enzyme has low activation temperature and will start prematurely at room temperature.
Kicki Bergefall
FAS Manager - Europe
Samples must reach 4°C and be held at this temperature for 5-10 minutes before proceeding directly to adaptor ligation.
Serena Pace
NGS Field Application Scientist, North America
