Tips include why you should proceed immediately to purification once incubation time is completed and advice for incubation.
Prepare reaction on ice since the enzyme has a low activation temperature and will start prematurely at room temperature.
Array and NGS Field Application Specialist, Europe
Use the proper master mix volume based on starting concentration.
NGS Field Application Scientist, North America
Do not use the heated lid during incubation.
Strategic Marketing Manager, SureSeq NGS
Proceed immediately to purification once incubation time is completed to prevent the ligation reaction continuing and generating adaptor dimers.
FAS Manager - Europe