Tips include why you should proceed immediately to purification once incubation time is completed and advice for incubation.
Prepare reaction on ice since the enzyme has a low activation temperature and will start prematurely at room temperature.
Pete Gray
Array and NGS Field Application Specialist, Europe
Use the proper master mix volume based on starting concentration.
Huiyan Jin
NGS Field Application Scientist, North America
Do not use the heated lid during incubation.
Serena Pace
NGS Field Application Scientist, North America
Proceed immediately to purification once incubation time is completed to prevent the ligation reaction continuing and generating adaptor dimers.
Kicki Bergefall
FAS Manager - Europe
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