Tips include how to prevent your chromosomes appearing distorted, how to maintain optimal results from hybridisation units, and advice if you are using an incubator for hybridisation.
Test ramp up and ramp down temperature times periodically as part of equipment QC on automated hybridisation units to ensure they are within manufacturer specifications.
Graham Halford
FISH Field Application Specialist, Europe
Maintaining humidity strips on automated hybridisation units according to manufacturer recommendations can help ensure optimal results from these units.
Ashley Hart
FISH Field Application Scientist, North America
Automated hybridisation units may fail to provide sufficient humidity, leading to poor hybridisation. Try using humidified chambers which can attain consistently higher humidity levels.
Laurence Cambridge
FISH Field Application Specialist, Europe
If your chromosomes appear distorted this may be because the coverslip was not sealed. In order to improve chromosome morphology, try sealing the coverslips prior to hybridisation.
Jenny Morse
FISH Field Application Scientist, North America
If you are using an incubator for hybridisation utilise a darkened, humidified chamber which has been pre-moistened (wet tissue) and pre-warmed.
Ashley Hart
FISH Field Application Scientist, North America
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