Tips include the benefit of using dedicated solution jars and how to produce sharper, more distinct probe signals by following one simple step.
Periodically washing solution jars and ensuring adequate detergent removal can help reduce background issues.
Strategic Marketing Manager, CytoCell FISH
Having dedicated solution jars (e.g. Coplin jars only for hot wash) for processing steps can prevent poor results due to contamination with incompatible solutions.
FAS Manager - North America
Have you tried placing counterstained FFPE slides in a -20°C freezer for minimum of 20 minutes after FISHing? Some labs say this helps to produce sharper, more distinct probe signals.
FISH Field Application Scientist, US & Canada