Tips include why you should use calibrated temperature-measuring devices and why you should test ramp up and ramp down temperature times periodically.
Use calibrated temperature-measuring devices to regularly QC hybridisation units; there may be significant slot to slot variability which can get worse over time leading to poor results.
Jenny Morse
FISH Field Application Scientist, North America
Test ramp up and ramp down temperature times periodically as part of equipment QC on automated hybridisation units to ensure they are within manufacturer specifications.
Ashley Hart
FISH Field Application Scientist, North America