First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest MM
  • Region 14q32.33
    20q12
  • Label    
  • Product No. LPH 077 (10 tests)
    LPH 077-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • MAFB, 20q12, Red

The IGH/MAFB Plus product consists of probes, labelled in green, proximal to the Constant, and within the Variable segment of the IGH region and MAFB probes (195kb, 161kb and 401kb), labelled in red. The MAFB probes are located on either side of the breakpoint region (between MAFB and PPP1R16B).

 

Probe information

The MAFB (MAF bZIP transcription factor B) gene is located at 20q12 and IGH (immunoglobulin heavy locus) at 14q32.33.

Approximately 50-60% of multiple myeloma (MM) cases are associated with translocations involving IGH and one of several partners including CCND1, NSD2 (MMSET) and FGFR3, CCND3, MAF or MAFB1.

The t(14;20)(14q32;q12) translocation is a recurrent translocation seen in around 2% of MMs2,3.

The reciprocal rearrangement brings a truncated form of the IGH μ-enhancer (Eμ, located between the joining (J) segments and the constant region of the IGH gene) in close contact with the MAFB gene4. The resultant fusion and the up-regulated transcription product has been shown to cause dysregulation of cyclin D21.

The prognostic outcome of t(14;20)(14q32;q12) is assumed to be the same as the t(14;16)(q32;q23)3.

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References

  1. Fonseca et al., Cancer Research 2004;64:1546-1558
  2. Fonseca et al., Leukemia 2009;23(12):2210-2221
  3. Sawyer, Cancer Genetics 2011;204(1):3-12
  4. Boersma-Vreugdenhil et al., Br J Haematol 2004;126:355-63

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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