First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest MM
  • Region 14q32.3
    16q23
  • Label    
  • Product No. LPH 101 (10 tests)
    LPH 101-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • MAF, 16q23, Red

The IGH/MAF v2 Translocation, Dual Fusion Probe consists of the IGH probe mix, labelled in green, covering parts of the Constant, J, D and Variable segments of the IGH gene and the MAF probe mix, labelled in red, that encompasses the MAF gene and flanking regions as well as the WWOX gene.

 

Probe information

MAF (MAF bZIP transcription factor) gene is located at 16q23 and IGH  (immunoglobulin heavy locus) at 14q32.3. Approximately 50-60% of multiple myeloma (MM) cases are associated with translocations involving IGH and one of several partners including CCND1, NSD2 (WHSC1) and FGFR3, CCND3, MAF or MAFB1. The t(14;16)(q32.3;q23) translocation is a recurrent translocation seen in 2-10% of MMs1.

The majority of the breakpoints occur within the last intron of WWOX (WW domain containing oxidoreductase), centromeric to MAF. These breakpoints have a dual impact of positioning the IGH enhancer near MAF and disrupting the WWOX gene2. Gene expression profiling of myeloma cell lines revealed that MAF caused transactivation of cyclin D2 (a promoter of cell cycle progression), thus enhancing proliferation of myeloma cells3.

According to the literature, MM patients harbouring the t(14;16) appear to have a more aggressive clinical outcome4,5.

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References

  1. Fonseca et al., Cancer Research 2004;64:1546-1558
  2. Walker et al., Blood 2013;121(17);3413-3419
  3. Chang H et al., Leukemia 2007;21:1572-1574
  4. Fonseca et al., Leukemia 2009;23(12):2210-2221
  5. Sawyer, Cancer Genetics 2011;204(1):3-12

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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