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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, Lymphoma
  • Region 8q24.21
    14q32.33
  • Label    
  • Product No. LPH 041 (10 tests)
    LPH 041-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • cMYC, 8q24.21, Red

The IGH/cMYC product consists of probes, labelled in green, covering the Constant and Variable segments of the IGH gene, and cMYC probes, labelled in red. The cMYC probe mix contains a 220kb probe centromeric to the cMYC (MYC) gene and a second probe covering the 186kb region telomeric to the cMYC gene, including the D8S1644 marker.

 

Probe information

The t(8;14)(q24;q32) translocation involving the IGH (immunoglobulin heavy locus) gene at 14q32.33 and the MYC (MYC proto-oncogene, bHLH transcription factor) oncogene at 8q24 is a recognised recurrent abnormality commonly seen in patients with B-cell malignancy.

IGH-MYC rearrangements are detected in up to 85% of cases of Burkitt lymphoma at diagnosis1. They are also seen in diffuse large B- cell lymphoma (DLBCL)2, multiple myeloma and plasmablastic lymphoma3,4.

In an IGH-MYC rearrangement the translocation breakpoints on chromosome 14 are clustered to a narrow region 5' to the intron enhancer of the immunoglobulin heavy chain, whereas the breakpoints on chromosome 8 can occur more than 340kb upstream of MYC, with no preferential site5. The translocation brings MYC into close proximity to the IGH enhancer and results in the up-regulation of MYC. Over-expression of the transcription factor stimulates gene amplification, resulting in uncontrolled cell proliferation6.

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References

  1. Perkins AS, Friedberg JW. Hematology Am Soc Hematol Educ Program. 2008;341-8
  2. Ott G, et al., Blood. 2013 Dec 5;122(24):3884-91
  3. Walker BA, et al., Blood Cancer J. 2014;4(3):e191
  4. Elyamany G, et al., Adv Hematol 2015;2015:315289
  5. Joos et al., Human Molecular Genetics 1992;1(8):625-32
  6. Erikson J et al., Proc Natl Acad Sci USA 1983;80(3):820-4

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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