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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest MPN
  • Region 4q12
  • Label    
  • Product Code LPH 032 (10 tests)
    LPH 032-S (5 tests)
  • Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.



Probe specification

  • FIP1L1, 4q12, Green
  • CHIC2, 4q12, Red
  • PDGFRA, 4q12, Green

The FIP1L1/CHIC2/PDGFRA product consists of a 177kb probe, labelled in green, located centromeric to the FIP1L1 gene, including the D4S1036 marker, a 174kb probe, labelled in red covering the CHIC2 gene and a 174kb probe, labelled in green, located telomeric to the PDGFRA gene, including the D4S956 marker.


Probe information

Deletion of CHIC2 (cysteine rich hydrophobic domain 2) at 4q12 results in the fusion of FIP1L1 (factor interacting with PAPOLA and CPSF1) at 4q12 with PDGFRA (platelet derived growth factor receptor alpha) at 4q12 producing a tyrosine kinase which transforms haematopoietic cells1.

In the 2008 World Health Organization (WHO) classification of myeloid neoplasms and acute leukaemia, a new subgroup of myeloid neoplasms was introduced: Myeloid and Lymphoid Neoplasms with Eosinophilia and Abnormalities of PDGFRA, PDGFRB or FGFR1. These neoplasms constitute three specific disease groups, with some shared features1.

The most common myeloproliferative neoplasms (MPN) showing PDGFRA rearrangements are those with FIP1L1-PDGFRA fusions. These MPNs present as chronic eosinophilic leukaemia (CEL) or, more rarely, as acute myeloid leukaemia (AML). As this abnormality is cytogenetically cryptic, FISH provides a useful tool for the detection of the fusion1,2.

Patients with the fusion benefit from treatment with tyrosine kinase inhibitors (TKIs). The diagnosis of the fusion gene can therefore lead to therapeutic choices for the patient1,2.

What our customers say


  1. Swerdlow et al., (eds,) WHO Classification of Tumours of Haematopoietic and Lymphoid Tissue, Lyon, France, 4th edition, IARC,2017
  2. Cools J et al., N Eng J Med 2003;348:1201-14

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

1. Sample & Slide Prep Dark Blue
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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