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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest AML, APL
  • Region 15q24.1
    17q21.1-q21.2
  • Label    
  • Product No. LPH 064 (10 tests)
    LPH 064-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • PML, 15q24.1, Red
  • RARα, 17q21.1-q21.2, Green

The PML probe mix, labelled in red, consists of a 151kb probe centromeric to the PML gene and a 174kb probe telomeric to the PML gene. The RARα probe mix, labelled in green, consists of a 167kb probe centromeric to the RARα (RARA) gene, including the CASC3 gene, and a 164kb probe, including the telomeric end of the RARα gene as well as the TOP2A and IGFBP4 genes.

 

Probe information

Immediate treatment of patients carrying the t(15;17) (PML/RARA) translocation is critical due to the risk of early death2. This FAST PML/RARA FISH probe allows rapid detection of the rearrangement, with only one hour of hybridisation required.

The fusion gene PML/RARA is created by the t(15;17)(q24.1;q21.2) translocation, found in 98% of AML M3 Acute hypergranular promyelocytic leukaemia and 9% of AML overall1,2,3. The PML protein is a transcription factor and RARA encodes a nuclear receptor. The fusion protein generated, PML-RARA, is a chimaeric transcription factor that operates as a dominant negative form of RARA and results in cells that are blocked at the promyelocytic stage of differentiation and then proliferate. Gain of function mutation allows repression of multiple genes and recruitment of DNA methyltransferases to promoters, allowing prolonged suppression. PML-RARA also activates components of the WNT signalling pathway, promoting stem cell renewal.

Immediate treatment of PML/RARA positive patients is critical as intravascular coagulation causes early death in 10-40% of cases2. Terminal differentiation is induced by the use of all-trans-retinoic-acid (ATRA), which reactivates the RARA gene and degrades the PML-RARA fusion product and 80-90% of treated patients achieve complete remission1,2,4. Despite the efficacy of ATRA, the death rate remains around 15-20%, but 70% of patients will achieve 3 year survival2. Additional abnormalities found with PML/RARA translocations include trisomy 8, seen in one third of cases, del(7q) and del(9q)2.

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References

  1. Licht, Sternberg, The Molecular Pathway of AML ASH Education Book 2005;137-42
  2. Huret and Chomienne. t(15;17)(q24;q21). Atlas Genet Cytogenet Oncol Haematol 1998;2(3):329-34
  3. Heim and Mitelman, Willey-Liss, Inc. 1995
  4. Greaves, BMJ 2002;324(7332):283-7

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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