First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest AML, MDS
  • Region 7q22.1-q22.2 / 7q31.2
  • Label    
  • Product No. LPH 025 (10 tests)
    LPH 025-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • 7q22.1-q22.2, Red
  • 7q31.2, Green

The 7q22 probe, labelled in red, covers a 396kb region including the telomeric end of the RELN gene and extending beyond the marker D7S658. The 7q31 probe, labelled in green, covers a 203kb region including the TES gene.

 

Probe information

Monosomy of chromosome 7 and deletions of the long arm of chromosome 7 are recognised recurrent chromosomal aberrations frequently seen in myeloid disorders.

Monosomy 7 and del(7q) are seen in a number of myeloid disorders, including myelodysplastic syndrome (MDS), acute myeloid leukaemia (AML), and juvenile myelomonocytic leukemia (JMML)1. Furthermore, it occurs in MDS and AML that develop in patients with constitutional disorders (eg, Fanconi anaemia, Kostmann syndrome, neurofibromatosis type 1, and familial monosomy 7)2. The presence of Monosomy 7 or del(7q) as karyotypic changes are associated with a poorer outcome in myeloid malignancies1,3.

Deletions of chromosome 7 are typically large with heterogeneity in the breakpoints in myeloid diseases, making it difficult to map the common deleted regions (CDRs). It is highly likely that multiple tumour suppressor genes on chromosome 7 cooperate in leukaemogenesis4. Two CDRs have been previously reported: one at 7q22 and the other on 7q31-q362,5, which are both targeted by this probe set.

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References

  1. Jerez et al., Blood 2012;119(25):6109-6118
  2. Fisher et al., Blood 1997;89(6):2036-2041
  3. Trobaugh-Lotrario et al., Bone Marrow Transplantation 2005;35(2):143-149
  4. McNerney et al., Blood 2013;121(6):975-983
  5. Thoennissen et al., American J Haem 2011;86(8):699-701

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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