First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest Lymphoma
  • Region 8q24.21
  • Label    
  • Product No. LPH 010 (10 tests)
    LPH 010-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • cMYC, 8q24.21, Red
  • cMYC, 8q24.21, Green

The cMYC probe mix consists of a 173kb probe labelled in red, centromeric to the MYC gene that includes the marker D8S1153 and a 186kb probe, labelled in green telomeric to the MYC gene that includes the D8S1644 marker.

 

Probe information

Chromosomal rearrangements involving the MYC (MYC proto-oncogene, bHLH transcription factor) gene at 8q24 are recognised recurrent abnormalities commonly seen in patients with B-cell malignancy.

MYC rearrangements, activating MYC by translocation with one of the three immunoglobulin loci (IGH, IGL or IGK), are detected in almost all cases of Burkitt lymphoma at diagnosis1. They are also seen in diffuse large B-cell lymphoma (DLBCL)2, multiple myeloma and plasmablastic lymphomas3,4, amongst other diseases.

MYC has also been shown on rare occasions to be involved in rearrangements with a number of non-immunoglobulin partners5.

The presence of concurrent MYC rearrangements with BCL2 and/or BCL6 rearrangements in patients with ‘dual-hit’ lymphoma has been shown to be associated with aggressive disease6.

What our customers say

References

  1. Perkins AS, Friedberg JW. Hematology Am Soc Hematol Educ Program. 2008;341-8
  2. Ott G, et al., Blood. 2013 Dec 5;122(24):3884-91
  3. Walker BA, et al., Blood Cancer J. 2014;4(3)
  4. Elyamany G, et al., Adv Hematol 2015;2015:315289
  5. Bertrand P, et al., Leukemia 2007;21:515-23
  6. Aukema SM, et al., Blood. 2011; Feb 24;117(8):2319-31

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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