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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, AML
  • Region 21q22.12
  • Label    
  • Product No. LPH 027 (10 tests)
    LPH 027-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • AML1, 21q22.12, Red
  • AML1, 21q22.12, Green

The AML1 probe mix consists of a 156kb probe, labelled in red, centromeric to the AML1 (RUNX1) gene that spans the CLIC6 gene and a 169kb probe, labelled in green, covering part of the AML1 (RUNX1) gene, including markers SHGC-87606 and D21S1921.

 

Probe information

The RUNX1 (RUNX family transcription factor 1) gene at 21q22.12 is one of the most frequent targets of chromosomal rearrangements observed in human acute leukaemia.

The most common rearrangements are the ETV6-RUNX1 and RUNX1-RUNX1T1 fusions. The ETV6-RUNX1 fusion is brought about by the t(12;21)(p13;q22) translocation, observed in around 21% of childhood B-cell acute lymphoblastic leukaemia (ALL) cases1, whilst the RUNX1- RUNX1T1 fusion is the result of the t(8;21)(q22;q22) translocation observed in 10-22% of patients with acute myeloid leukaemia (AML) FAB (French-American-British classification) type M2 and 5-10% of AML cases overall2,3. Both these rearrangements are considered good prognostic indicators in these diseases4,5.

The RUNX1 gene is also rearranged in many other rarer translocations, with partners including chromosomes 1, 2, 3, 4, 5, 6, 7, 9, 10, 14, 15, 16, 17, 18, 19, 20 and X6. This breakapart probe has been designed to allow the detection of rearrangements regardless of the partner gene.

Rearrangements of RUNX1 are not restricted to translocations. Using FISH, amplifications of chromosome 21 (iAMP21), including the RUNX1 gene, have also been found in childhood ALL7,8. These amplifications have been associated with poorer outcome9.

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References

  1. Jamil A et al., Cancer Genet Cytogenet 2000;122(2):73-8
  2. Swerdlow et al., (eds,) WHO Classification of Tumours of Haematopoietic and Lymphoid Tissue, Lyon, France, 4th edition, IARC,2017
  3. Reikvam H, et al., J Biomed Biotechnol. 2011; 2011:104631
  4. Shurtleff et al., Leukemia. 1995 Dec;9(12):1985-9
  5. Cho et al., Korean J Intern Med. 2003 Mar;18(1):13-20
  6. De Braekeleer et al., Anticancer Research 2009;29(4):1031-1038
  7. Niini T, Haematologica 2000;85(4):362-6
  8. Harewood et al., Leukemia. 2003 Mar;17(3):547-53
  9. Robinson HM et al., Leukemia 2003;17(11):2249-50

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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