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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, Lymphoma
  • Region 9p21.3
    9q12
  • Label    
  • Product No. LPH 009 (10 tests)
    LPH 009-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • P16, 9p21.3, Red
  • D9Z3, 9q12, Green

The P16 probe, labelled in red, covers a 193kb region of 9p21.3, extending from 105kb telomeric of P16 gene to 46kb centromeric of CDKN2B. The probe mix also contains a control probe for chromosome 9 (D9Z3, the heterochromatic block at 9q12) labelled in green.

 

Probe information

The CDKN2A (cyclin-dependent kinase inhibitor 2A) gene at 9p21 is a tumour suppressor gene that has been shown to be deleted in wide range of human malignancies.

Loss of the CDKN2A gene results in cellular proliferation and dysregulation of pro-apoptotic pathways. There are two proteins produced by the CDKN2A gene: p16INK4a and p14ARF, these protein products have been linked to two tumour suppressor pathways: the RB pathway and the p53 pathway, respectively1.

Deletions of 9p that include the CDKN2A gene are frequently reported in patients with acute lymphoblastic leukaemia (ALL): in approximately 30% of adult B-cell ALLs, 30% of childhood ALLs and up to 50% of T- cell ALLs. In adult B-cell ALL, CDKN2A deletions are frequently acquired in disease progression2,3,4,5.

Deletions including the CDKN2A locus have been reported in up to a third of patients with diffuse large B-cell lymphoma (DLBCL)6 and, in glioma, CDKN2A loss has been implicated with shorter overall survival in WHO grade I-III astrocytomas7.

Losses of the CDKN2A region have also been reported in malignant mesothelioma, melanoma, and bladder cancer8,9,10.

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References

  1. Møller MB, et al., Leukemia. 1999 Mar;13(3):453-9
  2. Moorman A V, et al., Blood. 2007;109(8):3189-97
  3. Sulong S, et al., Blood. 2014;113(1):100-7
  4. Schwab CJ, et al., Haematologica .2013 Jul;98(7):1081-8
  5. Xu N, et al., J Cancer. 2015;6(11):1114-20
  6. Jardin F, et al., Blood. 2010;116(7):1092-104
  7. Reis GF, et al., J Neuropathol Exp Neurol. 2015 May;74(5):442-52
  8. Conway C, et al., Genes Chromosomes Cancer. 2010 May;49(5):425-38
  9. Relan V, et al., PLoS One. 2013;8(3):e58132
  10. Stadler WM, et al., Clin Cancer Res. 2001;7(6):1676-82

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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