First slide
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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, CLL, Lymphoma
  • Region 6q23.3
    6p11.1-q11.1
  • Label    
  • Product No. LPH 016 (10 tests)
    LPH 016-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • MYB, 6q23.3, Red
  • D6Z1, 6p11.1-q11.1, Green

The MYB probe mix consists of a 183kb probe, labelled in red that covers the entire MYB gene and a region telomeric to the gene that includes a centromeric part of the AHI1 gene. This probe mix also contains a control probe for the 6 centromere (D6Z1) labelled in green.

 

Probe information

MYB (MYB proto-oncogene, transcription factor) at 6q23.3 is a transcription factor essential for haematopoiesis1.

The long arm of chromosome 6 (6q) is frequently involved in chromosomal abnormalities in human cancer, including haematological malignancies1. Deletions of chromosome 6q are found in acute lymphoblastic leukaemia (ALL), chronic lymphocytic leukaemia (CLL) and high grade nodal and extranodal B-cell lymphoma, but also in breast carcinoma, melanoma, ovarian carcinoma and renal cell carcinoma2,3.

Additionally, rearrangements involving MYB have been reported in T- ALL, for example the t(6;7)(q23;q34) translocation involving TRB seen in approximately 6% of patients, and focal duplications of the MYB locus, which are present in about 10% of patients1,4.

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References

  1. Clappier et al., Blood 2007;110(4):1251-1261
  2. Starostik et al., Blood 2000;95(4):1180-1187
  3. Stilgenbauer et al., Leukemia,1999;13:1331-1334
  4. Van Vlierberghe and Ferrando, J of Clin Inv 2012;122(10):3398-3406

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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