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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest Lymphoma
  • Region 22q11.21-q11.23
  • Label    
  • Product No. LPH 033 (10 tests)
    LPH 033-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGL, 22q11.21-q11.23, Red
  • IGL, 22q11.21-q11.23, Green

The IGL product consists of a 278kb probe, labelled in red, centromeric to the IGL Variable region and covering the MAPK1 gene, and a green probe, covering a 307kb region telomeric to the IGL Constant segment, including the BCR gene.

 

Probe information

Recurrent rearrangements involving the IGL (immunoglobulin lambda locus) gene at 22q11, with a wide range of partner genes, are seen in lymphomas and haematological malignancies.

A large number of B-cell malignancies harbour translocations involving the immunoglobulin (IG) loci. The majority of cases will show rearrangements involving the IGH gene; however, variant translocations have been described in 5-10% of B-cell neoplasms which involve either the immunoglobulin kappa (IGK) light chain locus at 2p11.2 or the immunoglobulin lambda (IGL) light chain locus at 22q111,2.

Variant translocations involving the IG light chain loci are seen in Burkitt lymphoma and multiple myeloma, with the presence of a t(2;8)(p12;q24) MYC-IGK, or t(8;22)(q24;q11) MYC-IGL3,5. In diffuse large B-cell lymphoma (DLBCL), translocations may involve the BCL6 gene via t(2;3)(p12;q27) or t(3;22)(q27;q11) translocations, or the BCL2 gene via t(2;18)(p12;q21) or t(18;22)(q21;q11) translocations6.

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References

  1. Poulseu TS et al., Leukemia 2002;16:2148-55
  2. Martin-Subero JI et al., Int J Cancer 2002;98:470-4
  3. Kornblau SM et al., Hematol Oncol 1991;9:63-78
  4. Walker BA, et al., Blood Cancer J; 2014;4(3):e191
  5. Chaganti SR et al., Genes Chromosomes Cancer 1998;23:323-7
  6. Tashiro S et al., Oncogene 1992;7:573-7

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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