The IGL product consists of a 278kb probe, labelled in red, centromeric to the IGL Variable region and covering the MAPK1 gene, and a green probe, covering a 307kb region telomeric to the IGL Constant segment, including the BCR gene.
Recurrent rearrangements involving the IGL (immunoglobulin lambda locus) gene at 22q11, with a wide range of partner genes, are seen in lymphomas and haematological malignancies.
A large number of B-cell malignancies harbour translocations involving the immunoglobulin (IG) loci. The majority of cases will show rearrangements involving the IGH gene; however, variant translocations have been described in 5-10% of B-cell neoplasms which involve either the immunoglobulin kappa (IGK) light chain locus at 2p11.2 or the immunoglobulin lambda (IGL) light chain locus at 22q111,2.
Variant translocations involving the IG light chain loci are seen in Burkitt lymphoma and multiple myeloma, with the presence of a t(2;8)(p12;q24) MYC-IGK, or t(8;22)(q24;q11) MYC-IGL3,5. In diffuse large B-cell lymphoma (DLBCL), translocations may involve the BCL6 gene via t(2;3)(p12;q27) or t(3;22)(q27;q11) translocations, or the BCL2 gene via t(2;18)(p12;q21) or t(18;22)(q21;q11) translocations6.
I first came across CytoCell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing CytoCell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time.
Medical Technologist, University of Arizona Health Network, USA