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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest Lymphoma, MM
  • Region 11q13.3
    14q32.33
  • Label    
  • Product No. LPH 045 (10 tests)
    LPH 045-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • MYEOV, 11q13.3, Red

The IGH/MYEOV product consists of probes, labelled in green, covering the Constant and Variable segments of the IGH gene, and MYEOV probes, labelled in red. The MYEOV probe mix contains a 155kb probe centromeric to the MYEOV gene, which includes the TPCN2 gene and a second probe, telomeric to the MYEOV gene, covering the 162kb region including the CCND1 and ORAOV1 genes.

 

Probe information

The MYEOV (myeloma overexpressed) gene is located at 11q13.3 and IGH (immunoglobulin heavy locus) at 14q32.33.

Approximately 50-60% of multiple myeloma (MM) cases are associated with translocations involving IGH and one of several partners including CCND1, NSD2 (WHSC1) and FGFR3, CCND3, MAF or MAFB1.

The t(11;14)(q13;q32) translocation is the most common translocation in MM, where it is seen in approximately 15% of cases2,3.

Unlike mantle cell lymphoma (MCL), where the breakpoints are clustered in a 1kb region that is 120kb centromeric to the CCND1 gene4, the breakpoints in MM cases are dispersed within a 360kb region between CCND1 and MYEOV at 11q135. MYEOV is a putative oncogene, located 360kb centromeric to CCND1, which is thought to be activated in the translocation by becoming closely associated with IGH enhancers. In contrast to IGH rearrangements in other neoplasms, those found in MM have IGH breakpoints predominantly in the C/J region, which, in the case of MYEOV, brings the MYEOV gene under the control of the 3' Eα1 enhancer5. In CCND1 translocations by contrast, the Eμ enhancer controls CCND1 expression. MYEOV overexpression is a possible prognostic factor in MM6.

The t(11;14)(q13;q32) associated with a favourable outcome in most series and therefore is regarded as neutral with regard to prognosis3.

What our customers say

References

  1. Fonseca et al., Cancer Research 2004;64:1546-1558
  2. Fonseca et al., Leukemia 2009;23(12):2210-2221
  3. Sawyer, Cancer Genetics 2011;204(1):3-12
  4. Ronchetti et al., Blood 1999 93(4):1330-1337
  5. Janssen et al., Blood. 2000 15;95(8):2691-2698
  6. Moreaux et al., Exp Haematol 2010;38(12):1189-1198

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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