The IGH/CCND1 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and CCND1 probes, labelled in red. The CCND1 probe mix contains a 155kb probe centromeric to CCND1 gene, covering a region between the D11S2663 and the D11S4095 markers, and a second probe (162kb) covering the telomeric end of CCND1 gene and the region up to FGF3 gene.
The t(11;14)(q13;q32) translocation involving CCND1 (cyclin D1) gene at 11q13.3 and the IGH (immunoglobulin heavy locus) gene at 14q32.33 is associated with mantle cell lymphoma.
The t(11;14)(q13;q32) rearrangement involving CCND1 and IGH is considered the hallmark of mantle cell lymphoma (MCL)1, the presence of which can be used to aid in the differential diagnosis of CD5+ B-cell lymphoproliferative disorders2.
I first came across CytoCell FISH probes in a previous lab I worked in and I was struck by the quality of the products. Since this time, I have been recommending and introducing CytoCell probes across all application areas — now they are the primary FISH probes used in our lab. They have an excellent range of products and their ready-to-use reagent format saves considerable time.
Medical Technologist, University of Arizona Health Network, USA