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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest CLL, Lymphoma
  • Region 11q13.3
    14q32.33
  • Label    
  • Product No. LPH 072 (10 tests)
    LPH 072-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • CCND1, 11q13.3, Red

The IGH/CCND1 Plus product consists of probes, labelled in green, proximal to the Constant, and within the Variable segment of the IGH region and CCND1 probes, labelled in red. The CCND1 probe mix contains a 155kb probe centromeric to CCND1 gene, covering a region between the D11S2663 and the D11S4095 markers, and a second probe (162kb) covering the telomeric end of CCND1 gene and the region up to FGF3 gene.

 

Probe information

The t(11;14)(q13;q32) translocation involving CCND1 (cyclin D1) gene at 11q13.3 and the IGH (immunoglobulin heavy locus) gene at 14q32.33 is associated with mantle cell lymphoma.

The t(11;14)(q13;q32) rearrangement involving CCND1 and IGH is considered the hallmark of mantle cell lymphoma (MCL)1, the presence of which can be used to aid in the differential diagnosis of CD5+ B-cell lymphoproliferative disorders2.

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References

  1. Vose JM. Am J Hematol. 2013;88(12):1082-8
  2. Ho AK, et al., Am J Clin Pathol 2009;131:27-32

Recommended protocol for CytoCell haematology FISH

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Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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