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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest Lymphoma
  • Region 14q32.33
    18q21.33
  • Label    
  • Product No. LPH 018 (10 tests)
    LPH 018-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • IGH, 14q32.33, Green
  • BCL2, 18q21.33, Red

The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and a 585kb probe, labelled in red, covering the BCL2 and KDSR genes.

 

Probe information

The t(14;18)(q32;q21) translocation involving the IGH (immunoglobulin heavy locus) gene at 14q32.33 and the BCL2 (BCL2 apoptosis regulator) gene at 18q21.33 is a recognised recurrent abnormality seen in B-cell malignancies.

IGH-BCL2 rearrangements are observed in 70-95% of follicular lymphoma (FL) cases and 20-30% of diffuse large B-cell lymphoma (DLBCL)1. Presence of the t(14;18) translocation in DLBCL is a predictor of outcome and has a poor prognostic effect2. BCL2 translocations have also been implicated in chronic B-cell lymphoproliferative disease (CLPD) and also occur occasionally in chronic lymphocytic leukaemia (CLL)3.

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References

  1. Tomita N., J Clin Exp Hematop 2011;51(1):7-12
  2. Barrans et al., Clin Cancer Res 2003; 9; 2133
  3. Bassegio L et al., Br J Haematol 2012;158(4):489-98

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

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