First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region 12q13.3
  • Label    
  • Product No. LPS 015 (10 tests)
    LPS 015-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • CHOP, 12q13.3, Red
  • CHOP, 12q13.3, Green

The CHOP Breakapart probe consists of a green 165kb probe and a red 146kb probe, which are positioned on each side of the CHOP (DDIT3) gene.

 

Probe information

Myxoid liposarcoma (MLS) is the most common subtype of liposarcoma1. The TLS-CHOP (FUS-DDIT3) t(12;16)(q13.3;p11) fusion gene, firstly described by Turc-Carel et al. in 19862, is now well established and is present in at least 95% of MLS3.

In rare cases, an EWS-CHOP (EWSR1-DDIT3) t(12;22)(q13;q12) translocation has been described4. The transcription factor gene, CHOP (DDIT3) (CREBP-homologous protein/DNA damage inducible transcript 3), is a negative regulator of adipocyte differentiation5.

The TLS (Translocated in Liposarcoma)6 or FUS7 gene is a nuclear RNA-binding protein with extensive sequence similarity to EWS. The TLS-CHOP protein interferes with adipocyte differentiation and favours proliferation over terminal differentiation4.

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References

  1. Enzinger and Weiss, Soft Tissue Tumors 3rd Ed. St Louis; MO:Mosby, 1995
  2. Turc-Carel et al., Can Genet Cytogenet 1986;23:291-9
  3. Mitelman, Catalog of chromosome aberrations in cancer. 5th Ed. 1995 New York: WileyLiss, Inc. 1994
  4. Antonescu et al., Clin Canc Res 2001;7:3977-87
  5. Hunag et al., Biol Open. 2012 Aug 15;1(8):705-10
  6. Crozat et al., Nature 1993;363:640-4
  7. Rabbitts et al., Nat Genet 1993;4:175-80

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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