CytoSure™ Genomic DNA Labelling Kits

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Efficient and reproducible labelling of DNA samples for use in aCGH.

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Details

The labelling of DNA samples used in array comparative genomic hybridisation (aCGH) is a critical step in the experimental process as poor labelling can result in inaccurate data.

OGT’s CytoSure Genomic DNA Labelling Kits have been uniquely developed and optimised to enable rapid delivery of high quality results with high signal-to-noise ratios.

CytoSure Genomic DNA Labelling Kits deliver:

  • Optimised formats to suit your throughput requirements
  • Reliable high quality results through higher signal intensity
  • Fast and simple procedure
  • Everything you need, from reagents to plasticware

Optimised formats to suit your throughput requirements

CytoSure Genomic DNA Labelling Kits are available in 2 formats to suit your throughput requirements. The CytoSure Genomic DNA Labelling Kit enables labelling of 24 samples and is ideal for labs running one or two arrays a week. The CytoSure HT Genomic DNA Labelling Kit is designed specifically for high-throughput labs and allows simultaneous labelling of 96 samples. This plate-based format offers faster sample processing, a lower cost per sample and decreased sample-to-sample variation using a master mix format. It is also easier to automate and track samples.

Our lab has tried a variety of different labelling methods; and found OGT's CytoSure Genomic DNA Labelling Kit to give consistently low DLRS values and excellent signal-to-noise ratios". Cytogenetics Laboratory, Baylor College of Medicine

Reliable high quality results

CytoSure Genomic DNA Labelling Kits have been rigorously tested with a wide range of sample types to ensure optimal performance. The unique formulation ensures superior signal-to-noise ratios allowing confident detection of copy number variation (Figure 1). This high signal-to-noise ratio means that even small aberrations can be reliably detected (Figure 2).

 

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Figure 1: High signal intensity allowing easy and accurate spot detection. Control DNA (Promega) was labelled using the CytoSure Genomic DNA Labelling Kit and another commercially available kit (Supplier A). The DNA labelled using the CytoSure Genomic DNA Labelling Kit exhibited far superior Cy3 signal intensity.

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Figure 2: Reliable detection of small aberrations. DNA labelled using the CytoSure Genomic DNA Labelling Kit was run on a CytoSure ISCA 8 x 60k array. CytoSure Interpret Software combined with high DNA signal intensity allowed detection of a small (130kb) DNA amplification.

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Fast and simple procedure

CytoSure Genomic DNA Labelling Kits offer much faster DNA labelling and clean up than traditional enzymatic labelling procedures. Labelling reactions using both the 24 and 96 reaction kits can easily be completed in a single day (Figure 3). The procedure can also be automated for implementation in high-throughput workflows.

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Figure 3: Two typical labelling workflows: With no need to digest, CytoSure Genomic DNA Labelling Kits save you at least 2 hours.

Everything you need, from reagents to plasticware

OGT offers the complete labelling solution — protocols, reagents, clean-up plates or columns and collection tubes. The unique kit formulation has been optimised for use with CytoSure arrays, which offer a wide variety of content and formats to suit your specific requirements. CytoSure arrays utilise 60-mer oligonucleotide probes, which are proven to offer higher signal-to-noise ratios and increased sensitivity and specificity1.

CytoSure products provide a complete and integrated solution for cytogenetics research in your lab — from DNA labelling and arrays to analysis software and full workflow automation.

For more information on CytoSure Genomic DNA Labelling Kits, please contact us.

Ordering information

ProductContentsCat. No. Price
CytoSure Genomic DNA Labelling Kit 24 reactions: dyes, nucleotide mix, random primers, enzyme, collection tubes 020020 Get a quote
CytoSure HT Genomic DNA Labelling Kit 96 reactions: nucleotide mix, random primers, enzyme 500040 Get a quote
Clean-up Columns 24 columns for the clean-up of DNA 500020 Get a quote
Purification Plate 96-well purification plate 500041 Get a quote

CytoSure™ products are for research use only; not for use in diagnostic procedures.

References

1. Curtis, C. et al (2009) The pitfalls of platform comparison: DNA copy number array technologies assessed. BMC Genomics 10, 588-610