Resources Brand Hero

Customer presentations

Genomic evaluation of CLL: Transitioning from a SNP-array CGH platform to a NGS-based CNV + SNV approach

Dr Fortin will talk about transitioning from microarray to the SureSeq™ CLL + CNV NGS panel, and how the SureSeq CLL + CNV NGS Panel delivers a comprehensive genomic profile for each CLL sample using a single workflow, including trisomy 12, 11q, 13q and 17p deletions.

Clinical evaluation of an NGS-based assay for the detection of CNV and SNV acting synergistically to affect phenotype

Dr McCready will examine the utility of a CytoSure® Constitutional NGS panel to identify relevant variants in families with reduced penetrance or uncertain CNVs. Findings from this pilot cohort were compared to data from chromosome microarray analysis to evaluate feasibility of the NGS platform for copy number detection and its utility in identifying SNVs acting synergistically with CNVs to affect phenotype.

Transitioning CLL FISH analysis into the molecular biomarker era

Dr. Tucker shares her experience of how OGT’s NGS panel, SureSeq™ CLL + CNV, has streamlined her laboratory workflow and enabled the detection of various aberrations in key genes implicated in CLL progression, including SNVs, indels, and CNVs. This alleviates the burden of running multiple assays and delivers comprehensive results with a single NGS analysis.

Analytical utility assessment of myeloid NGS gene panel testing; a single site experience

Dr McCready will compare a hybridization-based NGS panel versus single analyte and amplicon-based NGS assays for somatic mutation testing in myeloid malignancies, from her experience with OGT’s SureSeq myPanel™ Custom Myeloid - 49 gene plus panel.

Universal NGS Complete Workflow Solution protocol tutorials

NGS workflow – DNA fragmentation, end repair, and 3’ end A-tailing

Part I – Video illustrates the DNA fragmentation, end repair, and 3’ end A-tailing process in the Universal NGS Complete Workflow Solution.

NGS workflow – Adapter ligation

Part II - Video illustrates the adapter ligation process and the purification of the ligated library in the Universal NGS Complete Workflow Solution.

NGS workflow – Pre-capture PCR

Part III - Video illustrates the pre-capture PCR of the ligated library and its purification in the Universal NGS Complete Workflow Solution.

NGS workflow – Hybridization

Part IV - Video illustrates the hybridization process with design specific baits in the Universal NGS Complete Workflow Solution.

NGS workflow - Post-capture PCR, sample pooling, and sequencing

Part V – Video illustrates the post-capture PCR of the hybridized samples, and the final pooling for sequencing in the Universal NGS Complete Workflow Solution.

NGS library preparation protocol tutorials for solid tumor samples

NGS library preparation protocol for solid tumor samples - DNA fragmentation

This video illustrates the key steps for enzymatic DNA fragmentation prior to library preparation using the SureSeq™ NGS Library Preparation Kit for solid tumor samples.

NGS library preparation protocol for solid tumor samples - AMPure beads

This video illustrates the key steps for AMPure® purification using the SureSeq™ NGS Library Preparation Kit for solid tumor samples.

NGS library preparation protocol for solid tumor samples - Preparation of magnetic beads

This video illustrates the key steps for Dynabeads™ M270 Streptavidin magnetic bead preparation using the SureSeq™ NGS Library Preparation Kit for solid tumor samples.

NGS library preparation protocol for solid tumor samples – Hybrid capture

This video illustrates the key steps for hybrid capture using the SureSeq™ NGS Library Preparation Kit for solid tumor samples.

NGS library preparation protocol for solid tumor samples – Wash streptavidin beads

This video illustrates the key steps for post-hybridization bead washing using the SureSeq™ NGS Library Preparation Kit for solid tumor samples.

Interpret NGS Analysis Software tutorials

Introduction to Interpret NGS analysis software

Learn about Interpret, OGT’s powerful and easy-to-use next generation sequencing analysis solution, designed to work seamlessly with SureSeq and CytoSure NGS panels.

Interpret NGS analysis software - FASTQ Upload

Learn how easy it is to upload sample FASTQ files into OGT’s Interpret software and use them to create a batch for data analysis.

Interpret NGS analysis software - Viewing analysis batches

Learn how simply you can navigate round your analysis batch results and generate batch reports up front of variant analysis.

Interpret NGS analysis software – QC metrics

Learn how Interpret software creates easy to navigate displays of QC metrics to help users access the performance of their sequencing runs.

Interpret NGS analysis software – Analysis results by variants

Find out how to view analysis results by variants and explore using this feature in the Interpret software.

Interpret NGS analysis software – Variant results

Discover how to navigate and modify the Variant results page, selecting aberration types and customizing data analysis displays and tables with the many options available.

Conference presentations

Low frequency SNV and structural aberration detection in hematological disorders

Presented at the Festival of Genomics & Biodata 2022 conference, this presentation explores how OGT's SureSeq™ NGS panels allow accurate detection of low-frequency SNVs and indels, as well as structural aberrations such as ITDs, PTDs, CNVs, LOH and translocations.

Time to change: Transitioning from arrays to NGS in CLL & ID/DD analysis with confidence

Presented at the European Society of Human Genetics (ESHG) 2021 conference, discover how two scientists have streamlined their laboratory workflow using OGT’s NGS panels, allowing them to accurately detect a wide variety of genetic aberrations.

Transitioning CLL FISH analysis into the molecular biomarker era

Presented at the Cancer Genomics Consortium (CGC) 2021 conference, Dr. Tracy Tucker shares her experience in utilizing the SureSeq™ CLL + CNV NGS panel to transition her lab's CLL FISH analysis into the molecular biomarker era.

Time to change: Transitioning from arrays to NGS in CLL analysis with confidence

Presented at the American Cytogenetics Conference (ACC) 2021, this informative presentation explores transitioning from microarray to the SureSeq™ CLL + CNV NGS panel.

Painless transition from arrays to NGS in constitutional ID and DD analysis

Presented at the American College of Medical Genetics and Genomics (ACMG) 2021 conference, learn from one of OGT’s customers how utilizing CytoSure® Constitutional NGS panel assists in the detection of genetic modifiers in families with reduced penetrance or uncertain CNVs.

Implementing SureSeq Myeloid and CLL panels for comprehensive genomic profiling of hematological malignancies

Presented at the Association for Molecular Pathology (AMP) 2020 conference, this 50-minute workshop highlights two scientists’ experiences using OGT’s NGS panels in their hematology workflows, delivering comprehensive results with a single NGS analysis.

Webinars

Streamline your CLL CNV analysis and alleviate the burden of running multiple assays

In this webinar learn how the SureSeq™ CLL + CNV NGS Panel alleviates the burden of running multiple assays and streamlines your CLL research to deliver a comprehensive genomic profile for each CLL sample using a single NGS assay.

Painless transition from arrays to NGS in ID/DD analysis

Watch this webinar and learn how the CytoSure® Constitutional NGS solution, which delivers CNV analysis with excellent concordance with the ‘gold standard’ method, aCGH, can make the transition from arrays to NGS as painless as possible.

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