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Contributors

Lyudmila Georgieva1, Ezam Uddin1, Jacqueline Chan1, Faidra Partheniou1, and Graham Speight1

1Oxford Gene Technology (OGT), Oxford, UK

 

Introduction

  • Hybridisation-based enrichment protocols for next-generation sequencing (NGS) generate higher quality data (e.g. enhanced coverage uniformity, more complete coverage, and more accurate assessment of insertions/deletions (indels) and internal tandem duplications (ITDs)). However, they are generally more time consuming than PCR-based enrichment approaches. 
  • We have developed a rapid (30 minute) hybridisation protocol that enables Illumina sequencer-ready libraries to be generated from purified DNA in 1-day. 
  • The aim of this study is to evaluate the new streamlined 1-day hybridisation-based NGS library preparation kit (LPK) in conjunction with four different haematological capture panels of varying sizes.

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