The application of a one-day hybridisation-based enrichment protocol for NGS incorporating a rapid (30 minute) hybridisation step

Contributors

Lyudmila Georgieva¹, Ezam Uddin¹, Jacqueline Chan¹, Faidra Partheniou¹, and Graham Speight¹

¹Oxford Gene Technology (OGT), Oxford, UK

Introduction

• Hybridisation-based enrichment protocols for next-generation sequencing (NGS) generate higher quality data (e.g. enhanced coverage uniformity, more complete coverage, and more accurate assessment of insertions/deletions (indels) and internal tandem duplications (ITDs)). However, they are generally more time consuming than PCR-based enrichment approaches. 
• We have developed a rapid (30 minute) hybridisation protocol that enables Illumina sequencer-ready libraries to be generated from purified DNA in 1-day. 
• The aim of this study is to evaluate the new streamlined 1-day hybridisation-based NGS library preparation kit (LPK) in conjunction with four different haematological capture panels of varying sizes.