NGS Banner

Jacqueline Chan1 , Lyudmila Georgieva1 , Sabine Eckert1 , Faidra Partheniou1 and Graham Speight1

1OGT, Oxford, UK



  • Breast and Ovarian cancers are some of the most common cancers in women. 
  • Next-generation sequencing (NGS) has enabled the simultaneous study of mutations in high penetrance breast cancer predisposition genes. 
  • These include BRCA1BRCA2TP53PTEN, and PIK3CA, as well as more moderate risk genes such as PALB2BRIP1RAD51C and RAD51D.

Using OGT’s extensive background in bait design we have developed a range of fully tested and optimised baits targeting all coding exons of a range of key cancer-related genes (Table 1).

Table 1: Key breast and ovarian cancer-related genesTable 1: Key breast and ovarian cancer-related genes with empirically tested bait sets available in the SureSeq myPanel™ range.

To evaluate the application of a hybridisation-based approach we:

  • Compared the uniformity of coverage between a PCR amplification-based and the SureSeq™ hybridisation-based enrichment approach for BRCA1 and BRCA2 in solid tumour samples*. 
  • Assessed the performance of a custom panel (ALKKITEGFRKRAS, and TP53) from the SureSeq myPanel NGS Custom Cancer Panel range using the Quantitative Multiplex Reference Standard – gDNA and formalin-compromised DNA, from Horizon.

Register with us to read the full article

Once you have registered with us for free you will be able to read all our supportive literature, video tutorials and webinars.

  • Share
CTA Icon

Stay up-to-date with the latest news from OGT, including new products, support resources, and our DNA Dispatch newsletter