Example of normal breakapart signal pattern compared to abnormal signal pattern:
In some breakapart probes, the red and green signals may not be tightly co-localised in all cells, especially when chromatin is extended in interphase. It is therefore important to determine the cut-off levels for a normal result1.
- Analyse only intact nuclei, not overlapped or crowded nuclei or nuclei covered by cytoplasmic debris or high degree of auto-fluorescence.
- Signal intensity may vary, even within a single nucleus. In such cases, use single filters and/or adjust the focal plane.
1. Analysis and scoring guidelines recommended by The AGT Cytogenetics Laboratory Manual (fourth edition).