- Decrease the enzyme digestion time.
- After digestion, stain with DAPI and check under microscope.
- The number of over-digested cells should be <15% of the total.
Suggested digestion times:*
- Breast: 10-40 mins
- Lung: 15-20 mins
- Ovary: 10-20 mins
- Kidney: 20-25 mins
- Colon: 20 mins
- Brain: 15-18 mins
The optimal enzyme digestion time will depend on the age of the block, tissue composition and quality of fixation. Our technical support team are able to offer further help and guidance on optimising digestion times for your tissue.
*These timings are based on independent validation carried out by CytoCell on a representative set of tissues using the CytoCell Tissue Pretreatment Kit (LPS100) and should be used for guidance purposes only.