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  • Key
  • Problem
  • Identifier
  • Solution
Identifier
  • Protein contamination

 

Solution
  • Re-purify samples using proteinase steps
Low A260/280 values Image
Identifier
  • RNA contamination
Solution
  • Ensure that your DNA extraction protocol includes RNase
High A260/280 values Image
Identifier
  • Contamination of salts or solvents (e.g. Phenol)
Solution
  • Re-purified by ethanol precipitation
  • Resuspending the DNA in TE buffer
Low A260/230 values Image
Identifier
  • High DNA concentration (>350ng/μl)
Solution
  • Dilute DNA 1:2 in water or suitable buffer
Inaccurate sample concentration Image
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