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Product summary

  • Technology NGS
  • Application Haematology, Solid tumour
  • Gene Targets 1 - 140+
  • Aberration Types SNV, indel, ITD, PTD, CNV, translocation
  • Product No. Various
  • Intended use For research use only; not for diagnostic procedures.

Overview

Introduction - Tired of paying to sequence genes you’re not interested in? Then don’t!

SureSeq myPanel™ is a regularly updated, expert-curated library of pre-optimised cancer panel content for you to select from. Simply mix and match the gene, exonic or intronic content you need to create an NGS cancer panel that meets your exact requirements.

Our unique panel design coupled with hybridisation-based enrichment offers unparalleled coverage completeness and uniformity. This allows the accurate detection of difficult to sequence, low-frequency variants and minimises the requirement for supplementary fill-in with Sanger sequencing.

Getting started with your SureSeq myPanel NGS Custom Panel could not be simpler, find out more in this video…

Features
  • Hybridisation-based panel delivering unparalleled coverage uniformity — detect low-frequency variants consistently with confidence and minimise the requirement for supplementary fill-in with Sanger sequencing
  • Pre-optimised panels that meet your technical requirements and work with your samples (including FFPE tissue) — no more laborious in-house optimisation, decreasing assay development time
  • Bespoke panel content — sequence only what’s relevant for your cancer research, increase throughput and save on sequencing reagents
  • Panel content designed with experts and from current literature to target all relevant regions including intronic and splice sites — get the most comprehensive insight into disease-driving mutations

Gene targets

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Want to customise these gene targets?

A regularly updated, expert-curated library of pre-optimised cancer panel content for you to select from. Simply mix and match the gene, exonic or intronic content you need to create an NGS cancer panel that meets your exact requirements.

Capabilities

Excellent coverage of “difficult” genes

Mutations in the CEBPA gene are among the most common molecular alterations in AML and bear important significance. Sequencing of the CEBPA gene is often hampered by a repetitive nucleotide sequence and a high GC-rich content, which can lead to technical challenges in assay design, requiring a supplementary fill-in with Sanger sequencing. OGT’s expert bait design overcomes these issues, offer a high level of uniformity coverage for a notoriously difficult gene to sequence.

Don’t miss critical variants

The CALR gene is commonly mutated in various myeloproliferative neoplasms (MPNs). It is critical to identify key CALR indels (types 1 and 2 causing a frameshift) as well as increasingly recognised point mutations in this gene. OGT’s expert bait design offers excellent coverage uniformity across low-complexity regions with low nucleotide diversity (such as CALR exon 9), compared to other commercially available bait designs, which suffer from a considerable dip in coverage.

BCR-ABL fusion gene

The BCR-ABL gene fusion is formed following a balanced translocation of chromosome 9 and 22, generating the Philadelphia chromosome, a hallmark of CML. Combine SNV and indel detection with BCR-ABL translocation content and replace multiple assays with a single streamlined NGS workflow for a more comprehensive picture of all your samples.

Sophisticated bait design strategies allowing reliable KMT2A-PTD detection

Partial tandem duplications in KMT2A (MLL), frequently observed in AML, are notoriously difficult to detect due to their size, often spanning multiple exons. With OGT’s expertise in hybridisation-based panel design, your SureSeq myPanel offers robust detection of all sizes of KMT2A-PTDs, alleviating the burden of running multiple assays (Figure 5).

SureSample™

SureSample is a set of 44 single nucleotide polymorphisms (SNPs) to add to your SureSeq myPanel custom NGS panel to assist in sample identification. Throughout the library preparation process, they provide reliable sample tracking and easy identification of sample mix-up.

Whilst good practice in the handling of samples and increased laboratory automation minimises potential for error, additional checkpoints are valuable, particularly where multiple processing steps necessitate transfer of samples between different facilities.

The 44 SNPs selected have a minor allele frequency of 45-55% across six major HapMap populations, these 44 SNPs afford 88 points of allelic comparison providing highly discriminatory identifiers giving a likelihood of 1 in 5.6814 of two random samples having identical SNP profiles (Figure 4).

SureSample offers:

  • Confidence in results - SureSample provides an additional checkpoint valuable in supporting laboratory quality control
  • Reliable identification of sample mix-up - Maintain data integrity and sample provenance

SureSeq myPanel NGS Custom Cancer Panels workflow

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Content selection

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  • SureSeq myPanel NGS Custom Cancer Panels
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Sequencing

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  • Illumina sequencers

What our customers say

References

  1. Ross, David & Schafranek, Lisa & Hughes, Timothy & Nicola, Mario & Branford, Susan & Score, Joannah. (2009). Genomic translocation breakpoint sequences are conserved in BCR-ABL1 cell lines despite the presence of amplification. Cancer genetics and cytogenetics. 189. 138-9. 10.1016/j.cancergencyto.2008.10.010

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