CytoCell ZNF217 Amplification FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Breast cancer, Ovarian cancer, Sarcoma, Prostate cancer
Region 20q13.2
20p13
Label
Product Code LPS 005 (10 tests)
LPS 005-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

ZNF217, 20q13.2, Red
20pter, 20p13, Green

The ZNF217 Amplification probe consists of a 197kb red probe spanning the ZNF217 gene and neighbouring regions. The accompanying 20pter probe in green acts as a control for chromosome 20.

Probe information

The Zinc Finger protein 217 gene (ZNF217), which functions as a transcription repressor for a variety of genes¹, is a strong oncogene candidate mapped within the 260kb minimum common amplicon at 20q13.2².

ZNF217 is amplified in breast cancer^2,3, ovarian cancer and other tumours^3,4,5 and is associated with aggressive tumour behaviour⁶. It has been shown that the silencing of ZNF217 inhibits ovarian cancer cell growth and their invasive ability in cancer cell lines⁷.

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.