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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Breast cancer, Ovarian cancer, Sarcoma, Prostate cancer
  • Region 20q13.2
    20p13
  • Label    
  • Product No. LPS 005 (10 tests)
    LPS 005-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • ZNF217, 20q13.2, Red
  • 20pter, 20p13, Green

The ZNF217 Amplification probe consists of a 197kb red probe spanning the ZNF217 gene and neighbouring regions. The accompanying 20pter probe in green acts as a control for chromosome 20.

 

Probe information

The Zinc Finger protein 217 gene (ZNF217), which functions as a transcription repressor for a variety of genes1, is a strong oncogene candidate mapped within the 260kb minimum common amplicon at 20q13.22.

ZNF217 is amplified in breast cancer2,3, ovarian cancer and other tumours3,4,5 and is associated with aggressive tumour behaviour6. It has been shown that the silencing of ZNF217 inhibits ovarian cancer cell growth and their invasive ability in cancer cell lines7.

What our customers say

References

  1. Quinlan KG et al., Mol Cell Biol 2006;26:8159-72
  2. Collins C et al., Proc Natl Acad Sci 1998;95:8703-8
  3. Yang SH et al., Clin Cancer Res 2005;11:612-20
  4. Iwabuchi H et al., Cancer Res 1995;55:6172-80
  5. Bar-Shira A et al., Cancer Res 2002;62:6803-7
  6. Tanner MM et al., Clin Canc Res 1995;1:1455-61
  7. Sun G et al., Int J Oncol 2008;32:1065-71

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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