The ZNF217 Amplification probe consists of a 197kb red probe spanning the ZNF217 gene and neighbouring regions. The accompanying 20pter probe in green acts as a control for chromosome 20.
The Zinc Finger protein 217 gene (ZNF217), which functions as a transcription repressor for a variety of genes1, is a strong oncogene candidate mapped within the 260kb minimum common amplicon at 20q13.22.
ZNF217 is amplified in breast cancer2,3, ovarian cancer and other tumours3,4,5 and is associated with aggressive tumour behaviour6. It has been shown that the silencing of ZNF217 inhibits ovarian cancer cell growth and their invasive ability in cancer cell lines7.
We have successfully used CytoCell haematology probes over the last 5 years and were looking for the same quality and consistency for our FISH pathology screening. OGT worked closely with us to help our lab evaluate – and later validate – CytoCell pathology probes. CytoCell is now our primary FISH probe supplier. Results have been excellent and we were able to consolidate our workflow to follow a single, streamlined protocol.
Dr Mary Nordberg
Molecular Pathology Director, Delta Pathology Group, USA