The TOP2A Amplification/Deletion probe consists of a red 164kb probe spanning the TOP2A gene region. The centromeric probe in green acts as a control for chromosome 17.
The Topoisomerase 2A encoding (TOP2A) gene is located on chromosome band 17q21.2, near to the HER2 (ERBB2) oncogene. The TOP2A encoded protein has a function in DNA replication and the transcription of mRNA1,2. Amplification of the TOP2A gene is frequent in breast cancer and is often accompanied by HER2 amplification3.
TOP2A gene alterations are associated with an increased response to therapy using the anti-cancer drug anthracycline4, while patients with tumours that show normal levels of TOP2A and HER2 do not appear to gain any additional benefit from this drug4.
The quality and reproducibility of results using the CytoCell kit has been vital in accurately detecting co-deletions in our glioma investigations. We now have a cost-effective test that we can rely on that is also easy to use and interpret. We've been consistently impressed with this kit - not to mention the support offered by OGT's customer service, and have completely transitioned over to CytoCell probes.
Gavin Cuthbert, FRCPath
Head of Cancer Cytogenetics, Northern Genetics Service, Newcastle, UK