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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region Xp11.23
  • Label    
  • Product No. RU-LPS 051 (100μl)
    RU-LPS 051-S (50μl)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • TFE3, Xp11.23, Red
  • TFE3, Xp11.23, Green

The TFE3 Breakapart probe consists of two probes (279kb and 252kb), labelled in green, situated distal to the TFE3 gene and covering markers DXS6949 and STS-Z38762 and two probes (208kb and 325kb), labelled in red, situated proximal to the TFE3 gene and covering markers DXS9735 and DXS8366.

 

Probe information

TFE3 (transcription factor binding to IGHM enhancer 3) is a protein-coding gene located at Xp11.23. Recurrent rearrangements of the TFE3 gene have been reported in a number of neoplastic diseases – often grouped together as ‘Xp11 translocation cancers’ – these include: renal cell carcinoma (RCC), soft tissue alveolar soft part sarcoma (ASPS), perivascular epithelioid cell tumors (PEComa), epithelioid hemangioendotheliomas (EHE) and melanotic Xp11 translocation renal cancer1,2.

In RCC, TFE3 translocation partners have been shown to include the ASPSCR1, SFPQ and NONO genes3. Although RCC and ASPS have been shown to have identical ASPSCR1-TFE3 fusion transcripts, the t(X;17) translocation is consistently balanced in the former but usually unbalanced in the latter -  the derivative X chromosome is not seen in ASPS4.

In epithelioid hemangioendothelioma the novel YAP1-TFE3 fusion is seen, and defines a clinically distinct subset of this disease5,6, whereas in PEComa, the predominant partner has been shown to be the SFPQ gene7.

This research use only (RUO) probe has been designed for the investigation of TFE3 rearrangements, regardless of the partner gene involved.

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References

  1. Eble JN, et al., World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs. Lyon: IARC Press; 2004
  2. Wu A et al., Histopathology 2008;53(5):533-44
  3. Hodge JC, et al., Mod Pathol 2014 Jan;27(1):113–27
  4. Argani P, et al., Am J Pathol 2001;159(1):179–92
  5. Antonescu CR, et al., Genes, Chromosom Cancer. 2013 Aug;52(8):775–84
  6. Lee SJ, et al., Oncotarget. 2016;7(7):7480–8
  7. Rao Q, et al., Am J Surg Pathol. 2015 Sep;39(9):1181-96

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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