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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region 18q11.2
  • Label    
  • Product No. LPS 014 (10 tests)
    LPS 014-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • SYT, 18q11.2, Red
  • SYT, 18q11.2, Green

The SYT Breakapart probe consists of a green 151kb probe and a red 148kb probe, which are positioned on each side of the SYT gene.

 

Probe information

Synovial sarcomas account for up to 10% of soft-tissue sarcomas, typically arising in the para-articular regions in adolescent and young adults1.

A characteristic SYT(SS18)-SSX fusion gene resulting from the chromosomal translocation t(X;18)(p11;q11.2) is detectable in more than 90% of synovial sarcomas2, suggesting this is the primary causal event. The translocation fuses the SYT gene from chromosome 18q11.2 to either of two highly homologous genes at Xp11: SSX1 (Synovial Sarcoma X Breakpoint 1) or SSX2. In less than 1% of cases, SYT will be fused to a third gene, SSX43. SYT-SSX1 and SYT-SSX2 are thought to disrupt transcription and the subsequent expression of specific target genes4,5.

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References

  1. Enzinger FM, Weiss SW. Synovial sarcoma. In: Enzinger FM, Weiss SW, eds. Soft tissue tumors, 3rd ed. St. Louis: Mosby-Year Book, 1995:757–86
  2. Sreekantaiah et al., Am J Pathol 1994;144:1121-34
  3. Ladanyi et al., Canc Res 2002;62:135-40
  4. Ladanyi et al., Diagn Mol Pathol 1995;4:162-73
  5. Sorensen, Triche, Semin Cancer Biol 1996;7:3-14

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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