CytoCell SRD (CHD5) Deletion FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lymphoma, Breast cancer, Neuroblastoma, Glioma, Colorectal cancer, Gallbladder cancer, Gastric cancer, Laryngeal, Ovarian cancer, Lung cancer, Prostate cancer
Region 1p36.31
1q44
Label
Product Code LPS 010 (10 tests)
LPS 010-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

SRD (CHD5), 1p36.31, Red
1qter, 1q44, Green

The SRD (CHD5) Deletion probe consists of a 210kb red probe spanning the SRD (CHD5) gene region. The 1qter probe in green acts as a control for chromosome 1.

Probe information

The 1p36 region is frequently deleted in a broad range of human cancers¹. The Chromodomain Helicase DNA binding domain 5 (CHD5) gene acts as a tumour suppressor at 1p36.31 and is frequently deleted in human gliomas¹, leukaemias/lymphomas² and neuroblastomas³.

Deletion of the short arm of chromosome 1 is one of the most characteristic genetic changes in neuroblastoma, a tumour of the sympathetic nervous system. This is the most common childhood solid extracranial tumour, accounting for around 8%-10% of childhood cancers and 15% of childhood cancer deaths⁴.

The CHD5 gene has been characterised as the lead tumour suppressor candidate from the 1p36 smallest region of consistent deletion (SRD) region in neuroblastoma⁵.

What our customers say

It was very important for us to have more consistent results with our probes — easy-to-read bright signals and a range of vial sizes, which is much more cost-effective. It also was critical to upgrade our pretreatment kit to expedite the analysis of FFPE samples. We can now complete the process in about 90 minutes.

Janet Cowan, PhD

Director of the Cytogenetics Laboratory, Tufts Medical Center, USA

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FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.