First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma, Breast cancer, Neuroblastoma, Glioma, Colorectal cancer, Gallbladder cancer, Gastric cancer, Laryngeal, Ovarian cancer, Lung cancer, Prostate cancer
  • Region 1p36.31
    1q44
  • Label    
  • Product No. LPS 010 (10 tests)
    LPS 010-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • SRD (CHD5), 1p36.31, Red
  • 1qter, 1q44, Green

The SRD (CHD5) Deletion probe consists of a 210kb red probe spanning the SRD (CHD5) gene region. The 1qter probe in green acts as a control for chromosome 1.

 

Probe information

The 1p36 region is frequently deleted in a broad range of human cancers1. The Chromodomain Helicase DNA binding domain 5 (CHD5) gene acts as a tumour suppressor at 1p36.31 and is frequently deleted in human gliomas1, leukaemias/lymphomas2 and neuroblastomas3.

Deletion of the short arm of chromosome 1 is one of the most characteristic genetic changes in neuroblastoma, a tumour of the sympathetic nervous system. This is the most common childhood solid extracranial tumour, accounting for around 8%-10% of childhood cancers and 15% of childhood cancer deaths4.

The CHD5 gene has been characterised as the lead tumour suppressor candidate from the 1p36 smallest region of consistent deletion (SRD) region in neuroblastoma5.

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References

  1. Bagchi, Mills Canc Res 2008;68(8):2551-6
  2. Maser et al., Nature 2007;447:966-71
  3. Okawa et al., Oncogene 2008;27:803-10
  4. Broudeur and Maris, Neuroblastoma in Principles and Practice of Pediatric Oncology. 5th Ed. Philadelphia, PA: Lippincott: 933-970, 2006
  5. Fujita et al., J Natl Cancer Inst 2008;100:940-9

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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