First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma, Breast cancer, Neuroblastoma, Glioma, Colorectal cancer, Gallbladder cancer, Gastric cancer, Laryngeal, Ovarian cancer, Lung cancer, Prostate cancer
  • Region 1p36.31
  • Label    
  • Product Code LPS 010 (10 tests)
    LPS 010-S (5 tests)
  • Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.



Probe specification

  • SRD (CHD5), 1p36.31, Red
  • 1qter, 1q44, Green

The SRD (CHD5) Deletion probe consists of a 210kb red probe spanning the SRD (CHD5) gene region. The 1qter probe in green acts as a control for chromosome 1.


Probe information

The 1p36 region is frequently deleted in a broad range of human cancers1. The Chromodomain Helicase DNA binding domain 5 (CHD5) gene acts as a tumour suppressor at 1p36.31 and is frequently deleted in human gliomas1, leukaemias/lymphomas2 and neuroblastomas3.

Deletion of the short arm of chromosome 1 is one of the most characteristic genetic changes in neuroblastoma, a tumour of the sympathetic nervous system. This is the most common childhood solid extracranial tumour, accounting for around 8%-10% of childhood cancers and 15% of childhood cancer deaths4.

The CHD5 gene has been characterised as the lead tumour suppressor candidate from the 1p36 smallest region of consistent deletion (SRD) region in neuroblastoma5.

What our customers say


  1. Bagchi, Mills Canc Res 2008;68(8):2551-6
  2. Maser et al., Nature 2007;447:966-71
  3. Okawa et al., Oncogene 2008;27:803-10
  4. Broudeur and Maris, Neuroblastoma in Principles and Practice of Pediatric Oncology. 5th Ed. Philadelphia, PA: Lippincott: 933-970, 2006
  5. Fujita et al., J Natl Cancer Inst 2008;100:940-9

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

1. Heat Pretreatment Dark Blue
  • Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
  • Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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