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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lung cancer
  • Region 6q22.1
  • Label    
  • Product No. LPS 046 (10 tests)
    LPS 046-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • ROS1 Plus, 6q22.1, Green
    ROS1 Plus, 6q22.1, Red

The ROS1 Plus probe consists of 3 probes (175kb, 123kb, 171kb), labelled in green, situated proximal to the ROS1 gene and including markers D6S2428 and SHGC-149552 and a red probe (206kb) covering the distal part of the ROS1 gene and a region up to RH78348.

 

Probe information

The ROS1 (ROS proto-oncogene 1, receptor tyrosine kinase) gene at 6q22 is an ALK (anaplastic lymphoma receptor tyrosine kinase) gene paralogue which encodes a type I integral membrane protein with tyrosine kinase activity1.

ROS1 rearrangements define a molecular subset of non-small cell lung cancer (NSCLC) and are seen in approximately 2% of patients with NSCLC2. A number of partner genes have been identified, including SLC34A2, CD74 and SDC43. It has been shown that these ROS1 fusions activate the pSTAT3, PI3K/AKT/mTOR and SHP-2 phosphatase pathways4,5.

NSCLC patients with ROS1 rearrangements have been shown to respond to treatment with ALK/MET tyrosine kinase inhibitors, such as crizotinib6.

ROS1 rearrangements with the GOPC (golgi associated PDZ and coiled-coil motif containing) gene fusion partner were originally reported in glioblastoma, but have now also been detected on cholangiocarcinoma, ovarian cancer and NSCLC patient samples7-9.

The ROS1 Plus design covers the ROS1 region and the region deleted in ROS1-GOPC fusions.

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References

  1. Matsushime H et al., Mol Cell Biol 1986;6:3000-4
  2. Bergethon K et al., J Clin Oncol 2012;30(8):863-70
  3. Davies KD et al., Cancer Res 2012;72(8):1538-7445
  4. Birchmeier C et al., Proc Natl Acad Sci 1987;84:9270-9274
  5. Ou SH et al., Expert Rev Anticancer Ther 2012;12(4):447-56
  6. Shaw, A.T. et al., 2014. 371(21), pp.1963–71
  7. Gu TL et al., PLoS One 2011;6: e15640
  8. Charest A et al., Genes Chromosomes Cancer 2003;37:58-71
  9. Davies KD & Doebele RC, 2013. Clinical cancer 19(15), 4040–4045

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.

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