First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lung cancer
  • Region 10q11.21
  • Label    
  • Product No. LPS 045 (10 tests)
    LPS 045-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • RET,10q11.21,Green
  • RET,10q11.21,Red

The RET Breakapart probe consists of two probes (92kb, 303kb), labelled in green, situated proximal to the RET gene and covering markers G17488 and D10S1100 and two probes (351kb, 112kb), labelled in red, situated distal to the RET gene and covering markers D10S1176 and D10S2221.

 

Probe information

The RET (ret proto-oncogene) gene at 10q11 encodes for a transmembrane tyrosine kinase receptor involved in the control of cell differentiation, cell proliferation, and cell survival1.

Rearrangements involving the RET gene are recognised recurrent abnormalities seen in 1-2% patients with lung adenocarcinomas, where it is seen fused with KIF5B2,3, and papillary thyroid carcinoma where it is seen fused to a number of different partner genes including: CCDC6, PRKAR1A and NCOA44,5. The features of the proteins encoded by all types of RET fusion gene are similar to those of ALK: coiled-coil domains in the N-terminal fusion partners cause the RET domains to dimerize, resulting in activation of RET tyrosine kinase in the absence of ligands3.

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References

  1. Asai N, et al. Mol Cell Biol. 1995;15(3):1613–9
  2. Kohno T et al., Nat Med 2012;18(3):375-7
  3. Ju YS et al., Genome Res 2012;22(3):436-45
  4. Colato C, et al., Eur J Endocrinol. 2015;172(5):571–82
  5. Romei C et al., Front Endocrinol 2012;3:54

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.

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