CytoCell RB1 Deletion FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Breast cancer, Retinoblastoma, Lung cancer
Region 13q14.2
13q34
Label
Product Code LPS 011 (10 tests)
LPS 011-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

RB1, 13q14.2, Red
13qter, 13q34, Green

The RB1 Deletion probe consists of a 318kb red probe spanning the RB1 gene region. The 13qter probe in green acts as a control for chromosome 13.

Probe information

Retinoblastoma (Rb) is a cancer of immature retina cells that occurs in inf ants and small children¹. The protein encoded by the 180kb Retinoblastoma 1 (R B1) tumour suppressor gene, located on 13q14.2, may form complexes with oncoproteins and block their tumourigenic activity². Deletion of this gene, particularly homozygous deletion, is therefore a causal event in the development of retinoblastoma. The RB1 gene also plays a role in secondary tumours aris ing in retinoblastoma patients, such as osteosarcoma and some soft tissue sarcomas³. The RB1 gene may also be deleted as a result of a progressional tumourigenic event in some leukaemias⁴, as well as in some breast, lung, bladder, oesophagus and prostate cancers⁵.

FFPE tissue preparation and FISH protocol

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Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.