First slide
Cytocell Logo

Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest AML, APL
  • Region 17q21.1-q21.2
  • Label    
  • Product No. LPH 065 (10 tests)
    LPH 065 -S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • RARα, 17q21.1-q21.2, Red
  • RARα, 17q21.1-q21.2, Green

The RARα Breakapart probe mix consists of a 167kb probe centromeric to the RARα gene, including the CASC3 gene, and a 164kb probe, including the telomeric end of the RARα gene as well as the TOP2A and IGFBP4 genes.

 

Probe information

The PML (promyelocytic leukemia) gene is located at 15q24.1 and the RARA (retinoic acid receptor alpha) gene is located at 17q21.2. In the vast majority of acute promyelocytic leukaemia (APL) cases, the RARA gene at 17q21.2 fuses with PML gene at 15q24.1; however, in <5% of cases of APL, RARA is fused to alternative partner1.

Known variant fusion partners include NPM1 at 5q35, NUMA1 at 11q13, ZBTB16 (PLZF) at 11q23, STAT5B at 17q21, PRKARIA at 17q24, FIP1L1 at 4q12 and BCOR at Xp111,2,3.

Patients with variant RARA translocations may show variable sensitivity to treatment with some patients showing resistance to treatment protocols1,3. It is therefore important to differentiate between APL patients with PML-RARA fusion and those patients with variant RARA translocations.

This breakapart probe will detect rearrangements of the RARA gene, irrespective of partner genes or chromosomes involved.

What our customers say

References

  1. Creutzig et al., Blood 2012;120(16):3187-3205
  2. Zhang et al., Blood Reviews 2015;29(2):101-125
  3. Tomita et al., International Journal of Haematology 2013;97(6):717-725

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.

Request a quote for this product

FISH resources & support