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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Sarcoma
  • Region 1p36.13
  • Label    
  • Product No. LPS 013 (10 tests)
    LPS 013-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • PAX7, 1p36.13, Red
  • PAX7, 1p36.13, Green

The PAX7 Breakapart probe consists of a green 109kb probe and a red 139kb probe, which are positioned on each side of the PAX7 gene.

 

Probe information

Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma in children. One of the primary histological subtypes is the alveolar RMS (ARMS)1.

The majority (55%) of ARMS are associated with the t(2;13)(q36.1;q14) translocation, and less commonly (22%) with t(1;13)(p36;q14), leading to the fusion of transcription factor FOXO1 (Forkhead Box protein 1) to the transcription factors PAX3 (Paired Box protein 3) and PAX7, respectively2. The distinction between the t(1;13) and t(2;13) in ARMS is important as patients with the t(2;13) have a more adverse outcome than those with the t(1;13)3.

In ARMS cases that are negative for translocations involving FOXO1, there are two other variant PAX3 translocations that could be present - t(X;2)(q13;q36.1) PAX3-FOXO44 and t(2;2)(q36.1;p23) PAX3-NCOA15.

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References

  1. Kodet R et al., Am J Surg Pathol 1993;17(5):443-53
  2. Ahn et al., Oncol Rep. 2013 August; 30(2): 968–978
  3. Sorensen et al., J Clin Oncol 2002;20:2672-9
  4. Barr et al., Cancer Res 2002;62:4704-10
  5. Wachtel et al., Cancer Res 2004:64(16):5539-45

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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