First slide
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Product summary

  • Technology FISH
  • Application Solid tumour
  • Areas of interest Lymphoma
  • Region 17p13.1
    17p11.1-q11.1
  • Label    
  • Product No. LPS 037 (10 tests)
    LPS 037-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • P53, 17p13.1, Red
  • D17Z1, 17p11.1-q11.1, Green

The P53 probe is 161kb, labelled in red, covers the whole P53 (TP53) gene, extending 66kb telomeric to the gene and covering a region centromeric to the gene, to just beyond the marker D17S655. The probe mix also contains a control probe for the 17 centromere (D17Z1) labelled in green.

 

Probe information

Although previously difficult to detect, the advent of FISH analysis of interphase cells from patients with B-CLL showed that around 17% of patients with the disease have deletions of the P53 (TP53) gene1. As with ATM, deletions of P53 have important therapeutic implications for patients with B-CLL2.

Knowledge of the P53 deletion status in the patient should mediate the choice of therapy3. P53 is a tumour suppressor gene and its protein product is responsible for the death of cells that contain damaged DNA. This is thought to be brought about by phosphorylation of P53 and the subsequent prevention of its repression by MDM2 (Mouse Double Minute 2 Homolog). This phosphorylation is mediated by ATM. In the absence of P53 activity, cells that cannot be repaired by ATM will continue to proliferate in their damaged state. Patients deleted for P53 may be rendered resistant to alkylating chemotherapeutic agents3 and purine analogues4 as these are designed to damage DNA in the cells that P53 would have destroyed. In the absence of P53, therefore, patients treated with these agents will harbour a proliferating population of damaged cells.

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References

  1. Döhner et al., J Mol Med 1999;77:266-81
  2. Foá et al., Haematol 2013; 98(5):675-685
  3. Sturm et al., Cell Death Differ. 2003 Apr;10(4):477-84
  4. Döhner et al., Blood. 1995 Mar 15;85(6):1580-9

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat pretreatment Thumbnail
  • Heat 500ml Tissue Pretreatment Solution (Reagent 1) until it is either boiling or 96-98°C.
  • Heat slides for 15 minutes (Note: different incubation times may be required depending on tissue fixation. A 15 minute incubation is a recommended starting point).
  • Wash in PBS or dH2O at room temperature (RT) for 2 x 3 minutes.
Solid tumour FFPE FISH protocol Video Image
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Solid tumour FFPE FISH protocol

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