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Product summary

  • Technology FISH
  • Application Haematology
  • Areas of interest ALL, AML, CLL, Lymphoma, MDS, MM
  • Region 17p13.1
    17p11.1-q11.1
  • Label    
  • Product No. LPH 017 (10 tests)
    LPH 017-S (5 tests)
  • Intended use In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Chromomaps

Overview

Probe specification

  • P53, 17p13.1, Red
  • D17Z1, 17p11.1-q11.1, Green

The P53 probe mix consists of a 161kb probe, labelled in red that covers the whole P53 (TP53) gene and the flanking regions. The probe mix also contains a control probe for the 17 centromere (D17Z1) that is labelled in green.

 

Probe information

The TP53 (tumor protein p53) gene at 17p13.1 is a tumour- suppressor gene that has been shown to be deleted in a wide range of human malignancies.

The TP53 gene is one of most important tumour suppressor genes; it acts as a potent transcription factor with fundamental role in the maintenance of genetic stability. Screening for TP53 loss is important as deletions or losses of the short arm of chromosome 17, which includes the TP53 region, are reported in many cancers and are often associated with disease progression, inferior response to treatment and/or a poor prognosis.

In particular, loss of TP53 is reported in 10% of patients with chronic lymphocytic leukaemia (CLL), and is considered to be the poorest prognostic marker in that disease1,2. In acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL), TP53 loss is associated with a poor outcome and is often seen as a marker of disease progression or secondary disease3-5.

TP53 loss in patients with multiple myeloma is a late event, where is seen as a marker of disease progression and is associated with a very poor prognosis6,7.

In non-Hodgkin lymphoma, TP53 losses are reported in diffuse large B- cell lymphoma (DLBCL) often as part of ‘dual-hit’ lymphoma or plasmablastic phenotypes8. In mantle cell lymphoma (MCL), TP53 losses are associated with a poor outcome, and with a dismal outcome when seen with concurrent CDKN2A deletions9.

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References

  1. Rossi D, et al., Blood. 2013 Feb 21;121(8):1403-12
  2. Baliakas P, et al., Leukemia. 2014;(April):1-8
  3. Grimwade D, et al., Br J Haematol. 2010; (3):17
  4. Seifert H, et al., Leukemia. 2009;23(4):656-63
  5. Stengel A, et al., Blood. 2014;124(2):251-8
  6. Palumbo A, et al., J Clin Oncol. 2015 Sep 10;33(26):2863-9
  7. Fonseca R, et al., Leukemia. 2009 Dec;23(12):2210-21
  8. Simonitsch-Klupp I, et al., Leukemia. 2004 Jan;18(1):146-55
  9. Khayat AS, et al., BMC Gastroenterol. 2009;9:55

Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Sample and slide preparation Thumbnail
  • Spot the cell sample onto a glass microscope slide. Allow to dry.
  • Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
  • Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
  • Allow to dry.
Haematology FISH protocol Video Image
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Haematology FISH protocol

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